sem device  (JEOL)

Journal: Plant Methods

Article Title: FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques

doi: 10.1186/s13007-026-01510-z

Figure Lengend Snippet: Cryo-FE-SEM images of freeze-knife-fractured faces of young branches of ginkgo ( Ginkgo biloba ). Cryo-FE-SEM images of freeze-knife-fractured faces of young branches of gingko ( G. biloba ). A , B Low-magnification images. Boxed areas indicate the location of the phloem axial parenchyma cells, as shown in B – D . C Numerous cortical microtubules (CMTs) were observed on the protoplasmic fracture face (PF) of phloem axial parenchyma cells. The CMTs were generally oriented nearly perpendicular to the direction of cell elongation, with most exhibiting a meandering pattern across the cytoplasm. However, some CMTs were aligned nearly parallel to the direction of elongation. D High-magnification image of the PF. In regions where CMTs were bundled, ladder-like structures connecting adjacent microtubules were observed (boxed area). The ladder-like structures bridged two microtubules with an inter-microtubule spacing of approximately 20–40 nm. E , F In the PF of the epidermal parenchyma cells, the CMT bundles were arranged at an angle of approximately 45 ° relative to the direction of elongation. Each CMT bundle consisted of 3–4 microtubules. Black arrows = direction of cell elongation; White arrows = CMTs; CW = cell wall. Scale bars = 50 μm ( A ), 5 μm ( B ), 1 μm ( C , E ), 500 nm ( D , F )

Article Snippet: Observations were made using an FE-SEM device (JSM-7900F, JEOL) at an accelerating voltage of 1 kV, and the images were acquired using a secondary electron detector.

Techniques:

Journal: Plant Methods

Article Title: FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques

doi: 10.1186/s13007-026-01510-z

Figure Lengend Snippet: Cryo-FE-SEM images of freeze-knife-fractured calli of hybrid poplar ( Populus sieboldii × P. grandidentata ). Cryo-FE-SEM image of a freeze-knife-fractured face of the calli of hybrid poplar ( P. sieboldii × P. grandidentata ). A The cell surface exhibited a smooth, dome-like shape on the protoplasmic fracture face (PF). B On the PF, cortical microtubules (CMTs) were aligned nearly perpendicular to the longitudinal axis of the cell. C In regions where CMTs were densely bundled, each CMT bundle consisted of 4–6 microtubules. Black arrow = longitudinal axis of the cell; White arrows = CMTs; M = mitochondria. Scale bars = 3 μm ( A ), 1 μm ( B ), 500 nm ( C )

Article Snippet: Observations were made using an FE-SEM device (JSM-7900F, JEOL) at an accelerating voltage of 1 kV, and the images were acquired using a secondary electron detector.

Techniques:

Journal: Plant Methods

Article Title: FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques

doi: 10.1186/s13007-026-01510-z

Figure Lengend Snippet: Cryo-FE-SEM image of freeze-tensile-fractured surface of roots tips of adzuki beans ( Vigna angularis ). Cryo-FE-SEM images of a freeze-tensile-fractured face of the root tips of adzuki bean ( V. angularis ). A , B Both the protoplasmic fracture face (PF) and exoplasmic fracture face (EF) were reproducibly observed at the interfacial fracture of the lipid bilayer. Island-shaped depressions (white arrowheads) caused by the absence of the cell membrane were formed on the EF. C High-magnification image of the PF showing numerous cortical microtubules (CMTs) oriented approximately perpendicular to the direction of cell elongation. Occasionally, CMT bundles consisting of two microtubules were observed. D Enlarged image of EF shows cellulose microfibrils (CMFs) in areas where the cell membrane was absent (white arrowheads). The orientations of CMFs and CMTs were nearly identical. Black arrow = direction of cell elongation; White arrows = CMTs; CW = cell wall. Scale bars = 2 μm ( A ), 1 μm ( B ), 500 nm ( C , D )

Article Snippet: Observations were made using an FE-SEM device (JSM-7900F, JEOL) at an accelerating voltage of 1 kV, and the images were acquired using a secondary electron detector.

Techniques: Membrane

Journal: Plant Methods

Article Title: FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques

doi: 10.1186/s13007-026-01510-z

Figure Lengend Snippet: Tracing of cortical microtubules on the protoplasmic fracture face of root tips of adzuki bean ( Vigna angularis ). A Cryo-FE-SEM image of the protoplasmic fracture face (PF) from the root tips of adzuki bean ( V. angularis ). B Tracing of cortical microtubules (CMTs) on the PF, created based on the SEM image shown in A . The identification of CMTs was performed via visual confirmation. Black arrow = direction of cell elongation; Scale bar = 1 μm

Article Snippet: Observations were made using an FE-SEM device (JSM-7900F, JEOL) at an accelerating voltage of 1 kV, and the images were acquired using a secondary electron detector.

Techniques:

Journal: Plant Methods

Article Title: FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques

doi: 10.1186/s13007-026-01510-z

Figure Lengend Snippet: FE-SEM images captured at room temperature of freeze-tensile-fractured face of root tips of adzuki bean ( Vigna angularis ). FE-SEM images of a freeze-tensile fractured surface of the root tips of adzuki bean ( V. angularis ) captured at room temperature after freeze substitution and critical point drying treatment. A , B Low-magnification images. The boxed areas indicate the positions of the cells observed at high magnification in C – F . C , D A widespread protoplasmic fracture face (PF) with numerous cortical microtubules (CMTs). The exoplasmic fracture face (EF), which complementarily pairs with the PF observed via cryo-FE-SEM, was absent. E , F Enlarged views of the PF. The CMTs were oriented approximately perpendicular to the direction of cell elongation and their surfaces appeared to be covered by the protoplasmic half (P half) of the lipid bilayer. Island-like shapes of the ESs were also observed in some regions. Black arrow = direction of cell elongation; White arrows = CMTs; CW = cell wall. Scale bars = 100 μm ( A ), 10 μm ( B ), 3 μm ( C ), 1 μm ( D ), 500 nm ( E ), 200 nm ( F )

Article Snippet: Observations were made using an FE-SEM device (JSM-7900F, JEOL) at an accelerating voltage of 1 kV, and the images were acquired using a secondary electron detector.

Techniques:

Journal: Plant Methods

Article Title: FE-SEM visualization of cortical microtubules in plant cells using freeze-fracture techniques

doi: 10.1186/s13007-026-01510-z

Figure Lengend Snippet: Tracing of cortical microtubules on the protoplasmic fracture face of root tips of adzuki bean ( Vigna angularis ) after freeze substitution and critical point drying treatment. A FE-SEM image of the protoplasmic fracture face (PF) of the root tips of adzuki bean ( V. angularis ) captured at room temperature. B Tracing of cortical microtubules (CMTs) on the PF, created based on the SEM image shown in A . The identification of CMTs was performed via visual confirmation. Black arrow = direction of cell elongation; Scale bar = 1 μm

Article Snippet: Observations were made using an FE-SEM device (JSM-7900F, JEOL) at an accelerating voltage of 1 kV, and the images were acquired using a secondary electron detector.

Techniques: